Identifying GLP-1 analog oligomeric states with multi-angle light scattering

Identifying GLP-1 analog oligomeric states with multi-angle light scattering

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Overview

This application note addresses key questions in development and formulation of GLP-1 analogues such as liraglutide and semaglutide. It explores which oligomeric states are present and how the composition changes under different formulation conditions. Additionally, it investigates the presence of aggregates and how pH and ionic strength impact their formation. To answer all of these questions, multi-angle light scattering (SEC-MALS) was used to identify, quantify, and characterize the monomeric, oligomeric, and aggregate states of GLP-1 analogs under native and denaturing conditions.

Key Objectives

Native SEC-MALS: Identifies native oligomeric states and assesses their reversibility under varying formulation conditions.
Denaturing SEC-MALS: Confirms the monomeric state and quantifies residual dimers and trimers, supporting aggregate analysis.
Impact on Development: Facilitates understanding of aggregation pathways and supports robust formulation strategies to ensure safety and efficacy.
High molecular species identification: confidently state and distinguish HWMW vs the actual oligomer in solution!

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