Lipid nanoparticles (LNPs) are increasingly used in advanced drug delivery systems, particularly for genetic medicines and mRNA‑based therapeutics. As formulations grow more complex, understanding their physical stability and subvisible particle (SVP) content becomes critical for ensuring product quality, safety, and manufacturability. Traditional particle analysis techniques can struggle with complex colloidal and liposomal formulations, creating a need for a modern particle analysis approach that is suitable for LNP formulations.

In this application note, the Aura™ GT System uses Backgrounded Membrane Imaging (BMI) and Fluorescence Membrane Microscopy (FMM) to characterize stressed LNP formulations with sample volumes as low as 25 μL, demonstrating how freeze‑thaw stress, thermal stress, and buffer conditions influence particle formation and nucleic acid leakage.