Understanding Reversed Phase Selectivity for Different Compound Classes

Understanding Reversed Phase Selectivity for

Different Compound Classes

Brought to you by Phenomenex

Overview

Are you new to HPLC method development, or maybe you would welcome a quick refresh? This webinar will show you how to select an appropriate LC column based on the types of molecules you are looking to separate, and then highlight how choice of mobile phase can be optimized to help maximize selectivity. The focus of this webinar will be the reversed phase separation of small molecules, considering stationary phase interactions and the impact of mobile phase pH on retention, selectivity and peak shape.

Key Objectives:

Understand how to obtain good resolution by utilizing selectivity
Learn the basics of different stationary phase particle characteristics
Discover how to choose different stationary phases based on compound classes
Find how mobile phase can be used to change selectivity of columns

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