Scalable, Automated UF/DF for High-Concentration Monoclonal Antibody Manufacturing

DATE

September 16, 2025

TIME

8:00 a.m. PDT, 11:00 a.m. EDT, 16:00 BST, 17:00 CEST - Duration: 60 Minutes

Overview

High-concentration monoclonal antibody (mAb) formulations present major bioprocessing challenges, including increased viscosity, protein aggregation, and difficulties in process control. In this webinar, we demonstrate how process analytical technology (PAT) enables automated and optimized ultrafiltration/diafiltration (UF/DF) for high-concentration biologics, presenting a case study of the tangential flow filtration (TFF) KrosFlo® FS-15 RPM™ system with FlowVPX® in-line analytics.

Using a concentration–diafiltration–concentration (C/D/C) strategy, this method of integrating process analytics to your workflow delivers real-time monitoring, precise control, and improved accuracy—which mitigates risks associated with manual operation. Results presented show reliable performance at concentrations exceeding 200 g/L, with excellent comparability between in-line and at-line protein concentration measurements.

Key Learning Objectives:

Attendees will learn how automated tangential flow filtration (TFF) with RPM technology:

Maintains target protein concentrations and diafiltration volumes (DVs)
Reduces human error and manual calculation complexity
Enhances reproducibility and scalability in downstream processing
Accelerates development timelines for high-concentration biologics

Who Should Attend:

This webinar is ideal for scientists, process engineers, and biomanufacturing professionals working with monoclonal antibodies, high-concentration biologics, and downstream UF/DF processes. Join us to explore how automated, scalable TFF solutions can improve process control, reduce risk, and streamline biopharmaceutical manufacturing.

Brought to you by:

Speakers

Oleksandr Karpenko
Senior Field Applications Scientist,
Repligen

Kelly McSweeney
Contributing Editor,
C&EN Media Group

Registration

Forwarded this event? Clear the form

Email Address*

Salutation

First Name*

Last Name*

Company*

Job Title*

Address 1*

City*

State*

Zip / Post Code*

Country/Territory/Area*

Business Phone*

The FS-15 RPM™ System enables real-time control of high-concentration UF/DF processes to improve accelerate development. Would you like a Repligen specialist to contact you to learn more?*

Yes

I would like to receive C&EN’s weekly newsletter with the latest in chemistry news

I would like to receive more information on products and services from the American Chemical Society

address2

*By submitting this form, you agree to receive more information on related products and services from the American Chemical Society and its sponsor via email. You understand that you can withdraw your consent at any time. ACS takes your privacy seriously. For more information, please see the ACS Privacy Policy.

View On Demand Webinars