Shining Light on AAVs: Using Light Scattering Technologies to Solve Challenges in Characterization

October 18, 2023
8:00 a.m. PDT / 11:00 a.m. EDT / 16:00 BST / 17:00 CEST - Duration: 60 Minutes


With the rising clinical and commercial successes of AAV-based gene therapies, there is an ever-growing demand for robust, platform analytical technologies that can help ensure better product quality, safety, and efficacy. Size exclusion chromatography (SEC) and Field Flow Fractionation (FFF), coupled with multi-angle light scattering technologies (MALS), are rapidly emerging as transformative technologies for rapid and accurate characterization of AAV samples. Critical Quality Attributes (CQAs) such as capsid titer, genome titer, empty/full, % aggregation and purity of various AAV serotypes can all be obtained in a single measurement. This multi-attribute approach dramatically reduces operational costs and reduces drug development timelines.  

Key Learning Objectives:
  • See how SEC-MALS compares to orthogonal techniques (ddPCR, Capsid ELISA’s, and AUC) for the characterization of AAV during various downstream steps  
  • Understand how light scattering can be used as a rapid screening tool when working with multiple AAV serotypes 
  • Appreciate the value of FFF-MALS for accurate, AAV aggregation analysis  
Who Should Attend:
  • Academic researchers involved in biophysical studies 
  • Academic researchers involved in gene therapy studies 
  • Gene Therapy - Discovery 
  • Gene Therapy - Development
  • Gene Therapy – QC
  • Government researchers involved in gene therapy research

Brought to you by:
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Steven Milian, M.S.
Sr. Staff Scientist,
Research & Development Dept. of Patheon (by Thermo Fisher Scientific) Viral Vector Services
Gennarino Del Bagno, B.S.
Lead Scientist,
Research & Development Dept. of Patheon (by Thermo Fisher Scientific) Viral Vector Services
Sophia Kenrick, Ph.D.
Director of Analytical Sciences,
Waters|Wyatt Technology
Melissa O'Meara
Forensic Science Consultant,
C&EN Media Group


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