Direct Analysis of Glycosylated Therapeutics

DATE

October 19, 2023

TIME

9:00 a.m. PDT / 12:00 p.m. EDT / 17:00 BST / 18:00 CEST - Duration: 60 Minutes

Overview

Glycosylation plays a crucial role in determining the pharmacological properties of biotherapeutics. The molecular heterogeneity of glycosylated biotherapeutics makes it difficult to provide thorough characterization of intact glycoproteins by mass spectrometry. Current methods for analyzing biotherapeutics only measure fragments of the compound or rely on partial digestion of the glycans to reduce sample complexity. These limitations pose important risks and challenges for biotechnology companies that need to report human-compatible and consistent glycosylation, critical for drug safety and efficacy.

We will present a new approach to glycoform fingerprinting that uses proton-transfer charge-reduction with gas-phase fractionation to analyze intact glycoproteins by mass spectrometry. The method provides a detailed landscape of the intact molecular weights present in biotherapeutic protein preparations in a single experiment and offers insights into glycoform composition when coupled with a suitable bioinformatic strategy. In addition, we highlight the application of charge detection native mass spectrometry (Direct Mass Technology mode) towards the elucidation of masses and aggregation of heterogeneous glycosylated biotherapeutics.

Key Learning Objectives:

How to elucidate masses from heterogeneous glycosylated biotherapeutics
Learn how proton transfer charge reduction (PTCR) can simplify complex protein mixtures
Learn when to use PTCR based methods compared to Direct Mass Technology mode for analysis of biotherapeutics

Who Should Attend:

Laboratory Managers
Biopharmaceutical Researchers
Protein Scientists

Brought to you by:

Speakers

Wendy Sandoval
Director, Translational Mass Spectrometry,
Genentech

Ann Thayer
Contributing Editor,
C&EN Media Group

Registration

Forwarded this event? Clear the form

Email Address*

Salutation

First Name*

Last Name*

Company*

Job Title*

Address 1*

City*

State*

Zip / Post Code*

Country*

Business Phone

Yes, I would like a demo from Thermo Fisher Scientific

Yes, I would like to be contacted by a Thermo Fisher Scientific sales representative

Yes, I would like to receive email communications about Thermo Scientific products, events and special offers pertaining to my interests

I would like to receive more information on products and services from the American Chemical Society

*By submitting this form, you agree to receive more information on related products and services from the American Chemical Society (ACS Publications) and its sponsor via email. ACS takes your privacy seriously. For more information, please see the ACS Privacy Policy.

View On Demand Webinars