Innovative LC/MS Techniques for Oligonucleotide Analysis Without Ion Pairing Reagents

DATE

FIRST SESSION: January 29, 2025

SECOND SESSION: February 19, 2025

Can’t make it to the live event? Register and we will send you the link so you can watch on demand.

FIRST SESSION: January 29, 2025

8:00 A.M. PST, 11:00 A.M. EST, 4:00 P.M. GMT

Duration: 60 Minutes

SECOND SESSION: February 19, 2025

8:30 A.M. IST, 11:00 A.M. CST, 12:00 P.M. JST/KST

Duration: 60 Minutes

Overview

This webinar will delve into the limitations of traditional Ion Pairing Reversed Phase (IP-RP) chromatography, particularly its high costs, environmental impact, and need for specialized equipment. We will explore Hydrophilic Interaction Chromatography (HILIC) as a promising alternative, highlighting its compatibility with mass spectrometry (MS) and the absence of ion-pairing reagents, which offers greater flexibility in instrumentation.

Additionally, we’ll discuss recent advancements in ion pairing-free RP approaches for oligonucleotide analysis using LC/MS, showcasing studies on a range of oligonucleotides from 14mer to 103mer. Key parameters for optimizing LC/MS performance in these methods will also be covered.

Key Learning Objectives:

Understanding the HILIC retention mechanism and stationary phase selection to optimize oligo resolution and MS performance
Innovative reversed-phase separation free of ion-pairing reagents for MS characterization of nucleic acid therapeutics

Who Should Attend:

Oligonucleotide Analytical Method Development Scientists
Oligonucleotide Purification Method Development Scientists
Oligonucleotide Process Development Scientists
Oligonucleotide QC Scientists
Oligonucleotide CDMO Labs

Brought to you by:

Speakers

Connor Flannery
Global Product Manager HPLC Bio Columns
Agilent Technologies

Lee Bertram
Applications Scientist
Agilent Technologies

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