Advancing RNA Therapeutics: Mass Spectrometric Analysis of Antibody-siRNA Conjugates

Advancing RNA Therapeutics: Mass Spectrometric Analysis of Antibody-siRNA Conjugates

Brought to you by Agilent Technologies

Overview

Small interfering RNAs (siRNAs) are emerging as powerful tools for gene silencing in RNA-based therapeutics. To ensure effective delivery, siRNAs are often conjugated to monoclonal antibodies (mAbs), forming complex bioconjugates that require robust analytical characterization.

This application note introduces a native LC/MS workflow for analyzing intact mAb-siRNA conjugates. The method addresses limitations of traditional reversed-phase LC/MS by preserving conjugate integrity, offering a reliable approach for drug development and quality control.

Key Objectives

Understand the opportunities and challenges of developing siRNA-based drugs
Recognize the analytical challenges in characterizing mAb-siRNA conjugates
Get details about a novel LC/MS workflow developed for analysis of intact mAb-siRNA conjugates

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